Analisis Flavonoid Total Fraksi N-Heksan Ekstrak Biji Labu Kuning (Cucurbita moschata) Secara Spektrofotometri UV-VIS: Analysis of Total Flavonoids in the N-Hexane Fraction of Pumpkin Seed Extract (Cucurbita moschata) by UV-VIS Spectrophotometry

Istianatus Sunnah; Melati Aprilliana Ramadhani; Devi Mardiyanti

doi:10.35473/jhhs.v6i2.501

Authors

Istianatus Sunnah Universitas Ngudi Waluyo
Melati Aprilliana Ramadhani Universitas Ngudi Waluyo
Devi Mardiyanti Universitas Ngudi Waluyo

DOI:

https://doi.org/10.35473/jhhs.v6i2.501

Abstract

Infertility is one of the main health problems in Indonesia experienced by women with a percentage of 20-35%. One of the supporting therapies for infertility is the use of medicinal plants. One part of the plant that has many benefits in herbal medicine is pumpkin seeds (Cucurbita moschata). Pumpkin seeds contain flavonoid compounds, namely phytoestrogen isoflavones, which have similar properties to estrogen in the body. The purpose of this study was to analyze the total flavonoid content of pumpkin seeds as an initial stage in their activity as a fertility agent in women. The extraction method used was maceration using 96% ethanol, then the evaporation process was carried out, and the final stage was fractionation using ethyl acetate and n-hexane. Quantitative flavonoid tests were carried out on the n-hexane fraction using the UV-Vis spectrophotometry method. The results of the qualitative test of the n-hexane fraction of pumpkin seed extract were positive for flavonoids. Quantitative tests of the n-hexane fraction of pumpkin seed extract with 3 replications obtained total flavonoid levels, respectively, were 27.77; 27.15; and 27.36 mgQE/g. Pumpkin seeds contain non-polar flavonoids. The n-hexane fraction of pumpkin seed extract contains flavonoids with an average total flavonoid content of 27.43±0.32 mgQE/g.

ABSTRAK

Infertilitas merupakan salah satu permasalahan kesehatan utama di Indonesia yang dialami oleh wanita dengan persentase 20-35%. Salah satu terapi penunjang pada infertilitas dengan pemanfaatan tumbuhan yang berkhasiat obat. Salah satu bagian tumbuhan yang memiliki banyak manfaat dalam pengobatan herbal adalah biji labu kuning (Cucurbita moschata). Biji labu kuning mengandung senyawa golongan flavonoid yaitu isoflavon fitoestrogen yang memiliki kemiripan sifat dengan estrogen di dalam tubuh. Tujuan dari penelitian ini untuk menganalisis kadar total flavonoid biji labu kuning sebagai tahapan awal dalam aktivitasnya sebagai agen fertilitas pada wanita. Metode ekstraksi yang digunakan adalah maserasi menggunakan etanol 96%, kemudian dilakukan proses evaporasi, dan tahapan akhir dilakukan fraksinasi dengan menggunakan etil asetat dan n-heksan. Uji kuantitatif flavonoid dilakukan pada fraksi n-heksan menggunakan metode spektrofotometri UV-Vis. Hasil uji kualitatif fraksi n-heksan ekstrak biji labu kuning adalah positif mengandung flavonoid. Uji kuantitatif fraksi n-heksan ekstrak biji labu kuning dengan 3 kali replikasi didapatkan kadar flavonoid total berturut-turut adalah 27,77; 27,15; dan 27,36 mgQE/g. Biji labu kuning memiliki kandungan flavonoid non polar. Fraksi n-heksan ekstrak biji labu kuning mengandung flavonoid dengan kadar flavonoid total rata-rata 27,43±0,32 mgQE/g.

Downloads

Download data is not yet available.

References

A. Makuasa, D. A., & Ningsih, P. (2020). The Analysis of Total Flavonoid Levels In Young Leaves and Old Soursop Leaves (Annona muricata L.) Using UV-Vis Sepctrofotometry Methods. Journal of Applied Science, Engineering, Technology, and Education, 2(1), 11–17. https://doi.org/10.35877/454ri.asci2133

Abu bakar, A., & Haque, M. (2020). Preparation of Medical Plants : Basic Extraction and Fractionation Procedures for Experimental Purposes. Journal of Pharmacy and Bioallied Sciences, 12, 1–10. https://doi.org/10.4103/jpbs.JPBS

Akbaribazm, M., Goodarzi, N., & Rahimi, M. (2021). Female infertility and herbal medicine: An overview of the new findings. Food Science and Nutrition, 9(10), 5869–5882. https://doi.org/10.1002/fsn3.2523

An Gai Hong, G., Chen, X. W., Li, C., Zhang, L., Wei, M. F., Chen, J. J., Ma, Q., Yang, D. F., & Wang, J. (2020). Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress. BioMed Research International, 2020. https://doi.org/10.1155/2020/4701563

Anand David, A. V., Arulmoli, R., & Parasuraman, S. (2016). Overviews of biological importance of quercetin: A bioactive flavonoid. Pharmacognosy Reviews, 10(20), 84–89. https://doi.org/10.4103/0973-7847.194044

BPOM RI. (2023). Pedoman Penyiapan Bahan Baku Obat Bahan Alam Berbasis Ekstrak / Fraksi. Badan Pengawas Obat Dan Makanan RI, November, 45.

Doloking, H., Tahar, N., Mukhriani, & Surya Ningsi. (2022). Flavonoids: A Review On Extraction, Identification, Quantification, and Antioxidant Activity. Ad-Dawaa’ Journal of Pharmaceutical Sciences, 5(1). https://doi.org/10.24252/djps.v5i1.29329

Goti, D., & Dasgupta, D. S. (2023). A comprehensive review of conventional and non-conventional solvent extraction techniques. Journal of Pharmacognosy and Phytochemistry, 12(3), 202–211. https://doi.org/10.22271/phyto.2023.v12.i3c.14682

Hagos, M., Chandravanshi, B. S., Redi-Abshiro, M., & Yaya, E. E. (2023). Determination of Total Phenolic, Total Flavonoid, Ascorbic Acid Contents and Antioxidant Activity of Pumpkin Flesh, Peel and Seeds. Bulletin of the Chemical Society of Ethiopia, 37(5), 1093–1108. https://doi.org/10.4314/bcse.v37i5.3

Kementerian Kesehatan RI. (2017). Farmakope Herbal Indonesia. https://doi.org/10.2307/jj.2430657.12

Mammen, D., & Daniel, M. (2012). A critical evaluation on the reliability of two aluminum chloride chelation methods for quantification of flavonoids. Food Chemistry, 135(3), 1365–1368. https://doi.org/10.1016/j.foodchem.2012.05.109

Motamed Jahromi, S., & Niami Jahromi, S. (2020). The Effect of Hydro-Alcoholic Extract of Pumpkin Seeds on Estrogen Levels and Kidney Markers in Adult Female Rats. Iranian Red Crescent Medical Journal, 22(4). https://doi.org/10.5812/ircmj.98864

Pękal, A., & Pyrzynska, K. (2014). Evaluation of Aluminium Complexation Reaction for Flavonoid Content Assay. Food Analytical Methods, 7(9), 1776–1782. https://doi.org/10.1007/s12161-014-9814-x

Ramos, R., Bexerra, I., Ferreira, M., & Soares, L. A. (2017). Spectrophotometric Quantification of Flavonoids in Herbal Material, Crude Extract, and Fractions from Leaves of Eugenia uniflora Linn. Pharmacognosy Research, 9(3r). https://doi.org/10.4103/pr.pr

Rasheed, N., Ahmed, A., Nosheen, F., Imran, A., Islam, F., Noreen, R., Chauhan, A., Shah, M. A., & Amer Ali, Y. (2023). Effectiveness of combined seeds (pumpkin, sunflower, sesame, flaxseed): As adjacent therapy to treat polycystic ovary syndrome in females. Food Science and Nutrition, 11(6), 3385–3393. https://doi.org/10.1002/fsn3.3328

Sabarudin, Ruslin, Zubaydah, W. O. S., Sartinah, A., Buton, S., & Yamin. (2021). Antiradical activity, total phenolic, and total flavonoids extract and fractions of pumpkin (Cucurbita moshata Duch) leaves. Food Research, 5(2), 348–353. https://doi.org/10.26656/fr.2017.5(2).529

Shaikh, J. R., & Patil, M. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603–608. https://doi.org/10.22271/chemi.2020.v8.i2i.8834

Shraim, A. M., Ahmed, T. A., Rahman, M. M., & Hijji, Y. M. (2021). Determination of total flavonoid content by aluminum chloride assay: A critical evaluation. Lwt, Food Science and Technology, 150(111932). https://doi.org/10.1016/j.lwt.2021.111932

Skoracka, K., Ratajczak, A. E., Rychter, A. M., Dobrowolska, A., & Krela-Kaźmierczak, I. (2021). Female Fertility and the Nutritional Approach: The Most Essential Aspects. Advances in Nutrition, 12(6), 2372–2386. https://doi.org/10.1093/advances/nmab068

Sunnah, I., Fadiyah, G. A., & Octavia, P. A. (2023). Karakteristik Fisik Dan Formulasi Minyak Biji Labu Kuning (Cucurbita Moschata D) Sebagai Body Scrub.pdf. Media Informasi Penelitian Kabupaten Semarang, 5(1), 392–400.

Ullah, A., Munir, S., Badshah, S. L., Khan, N., Ghani, L., Poulson, B. G., Emwas, A., & Jaremko, M. (2020). Important Flavonoids and Their Role as aTherapeutic Agent. Molecules, 25, 1–39.

Venturella, R., Vaiarelli, A., Cimadomo, D., Pedri, S., Lico, D., Mazzilli, R., Mocciaro, R., Rienzi, L., Di Carlo, C., Ubaldi, F. M., & Zullo, F. (2019). State of the art and emerging drug therapies for female infertility. Gynecological Endocrinology, 35(10), 835–841. https://doi.org/10.1080/09513590.2019.1603289

Zhuang, B., Ramanauskaite, G., Koa, Z. Y., & Wang, Z. G. (2021). Like dissolves like: A first-principles theory for predicting liquid miscibility and mixture dielectric constant. Science Advances, 7(7), 1–8. https://doi.org/10.1126/sciadv.abe7275